career objective                              

results-driven biotech scientist eager to contribute extensive expertise in molecular biology and bacteriology toward supporting a progressive organization seeking a motivated and productive team member

profile                                                

ø  offer numerous years of biotechnology experience with expertise in dna manipulation, recombinant gene (antigen gene) design and expression, bacterial strain and gene expression vector construction, recombinant protein and native protein purification, and in vitro/in vivo vaccine evaluation

ø  develop ground-breaking methods and processes that result in realistic solutions and new products; consistently achieve critical schedule and budget targets while maintaining high quality of work

ø  keen eye for detail and emphasis on accurate documentation;  dynamic communication and presentation skills

ø  proactive team player equally effective in independent and collaborative environments; strong supervisory experience

 

~  proficiencies ~

nucleic acid manipulation: dna and rna purification; primer design; recombinant gene design; pcr;  southern blot;  northern blot; gene cloning; dna sequencing and analysis; blast search; gene knockout; gene mutagenization; gene fusion and split

gene expression and protein purification: expression of native genes and variety of recombinant genes in e-coli and other systems; purification of native and recombinant proteins; protein detection and characterization; sds-page; protein denature and refold

bacteriology: cultivation of both aerobic and anaerobic bacteria; isolation of bacteria from variety of environments including clinic samples, plant tissues, water and soils; identification and characterization of bacteria;  working experience with  e coli, agrobacterium spp., clostridium perfringens, salmonella cholreasuis, mycoplasma hyopneumoniae, and bacillus thuringiensis

immunology: antigen identification and characterization with western blot, ifa, immunoprecipitation; evaluation of potential vaccines and vaccines in vivo and in vitro

cell cultures: cell invasion, transfection, and culture; cell line maintenance; detection of protein expression on cell surfaces

virology:  virus isolation, propagation, identification, titration, and recombinant virus reconstitution

professional experience                

department of research & development, boehringer ingelheim vetmedica, inc.                                       11/03 c present

scientist iii (1/06 c present)

• in recognition of outstanding performance, progressed within organization to attain position entrusted with broad range of r&d responsibilities including supervisory and project management duties; utilize problem-solving abilities to contribute to product development
• lead and participate in innovative animal health improvement projects; successfully developed a promising, live bacterial vaccine via gene delivery system resulting in a 50% reduction in cost of protein production; created a mycoplasma vaccine by cloning and optimizing gene code
• actively developed new dna manipulation method that reduces number of cloning steps
• proficiently created and investigated infectious clones from a vaccine and a virulent strain, performed in vivo evaluation to obtain a potential vaccine clone; created reconstituted virus in mammalian cells for evaluation as a potential vaccine
• diligently document all activity, compile and analyze data to generate reports and presentations instrumental to senior management in assessing performance and determining course of product development

postdoctoral associate (11/03 c 12/05)

• effectively identified and patented two antigen genes from a pathogen bacterium and constructed a bacterial strain as an antigen gene delivery vector
• developed systems for high throughout gene expression and cloning used for more than 50 genes; avoided optimization of expression conditions, using restriction enzymes, and ligation mediated by ligase
• created recombinant protein purification system that simplifies and expedites procedures while improving protein purity

 

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department of microbiology, university of washington, seattle                                                               4/02 c 10/03

postdoctoral associate

         studied the interaction of agrobacterium tumefacience and its host plants through gene deletion, gene knockout, and gene regulation

         set up a markerless knockout system and designed a system to detect tumorigenesis

 

institute of plant protection, chinese academy of agricultural sciences, beijing                                       1/93 c 12/95

research scientist

         isolated bacillus spp. from the leaf surfaces of vegetables; identified bacillus thuringiensis strains for biological control of insects

 

department of biology, beijing normal university                                                                                   7/88 c 12/92

assistant professor

         created course content and conducted undergraduate microbiology classes

 

~ patent ~

title: lawsonia intracellularis immunological proteins. usa no. 11/414764                                                2006

education                                           

kyoto university, japan
ph.d., molecular biology                                                                                                                     2001

m.s., molecular biology                                                                                                                       1998

 

beijing agricultural university, china

m.s., microbiology and plant pathology                                                                                              1988

b.s., microbiology and plant pathology                                                                                               1988

selected publications                

• wang, yue, yyyyyy x. yyyyyy et al. (2008) attenuation of porcine reproductive and respiratory syndrome virus strain mn184 using chimeric construction with vaccine sequence. virology,  371(2):418-429

 

• liang, yajie, takashi aoyama et al. (1998) structural characterization of the virb operon on the hairy-root-inducing plasmid a4. dna research 5(2) 87-93

 

• wang, huimin, yyyyyy x. yyyyyy et al. (1995) control of the crown gall diseases on stone fruit trees with anti-gall agent. plant protection. 21(1):24-26

 

• ph.d. dissertation -- title: determination of genomic dna sequences and structural characterization of plasmid pria4 of agrobacterium rhizogences.  (as a result of technical problems, this work has not been published yet.)