career objective                              

recent biochemistry and molecular biology phd. graduate eager to contribute exceptional laboratory research skills towards actively supporting a progressive organization in achieving its objectives

profile                                                

ø  offer more than 6 years of biology / biochemistry experience, with expertise in protein field research

ø  solid background in employing established laboratory techniques, conducting research, collecting and analyzing data, and preparing reports instrumental to the success of key projects

ø  provide comprehensive support for senior scientists, researchers, and project managers

ø  consistently achieve critical schedule and budget targets while maintaining high quality standards; keen eye for detail and emphasis on accurate documentation

ø  proactive team player equally effective in independent and collaborative environments; dynamic research, communication, and presentation skills

ø  sharp technical proficiencies including dsc, itc, maldi-tof mass spectrometry, microarray, stopped flow analysis, epr, and uv/vis spectroscopy

ø  fully bilingual in english / chinese

~ core competencies ~

biophysical assays ~ dna plasmid purification ~ enzymatic kinetics ~ fplc ~ integral membrane protein purification and characterization ~ membrane protein crystallization ~ molecular cloning ~ pcr ~ protein binding assay ~

protein expression ~ protein purification ~ sds-page ~ site-directed mutagenesis ~ western blot

education                                           

ph.d. in biochemistry and molecular biology, oklahoma state university, stillwater, ok                       december 2009

• dissertation title: the role of protein subunits in superoxide generation by the cytochrome bc1 complex from rhodobacter sphaeroides.  advisor: dr. chang-an yu
• attended proteomics & mass spectrometry and microarray workshops
  
  

b.s. in biotechnology, sichuan university, chengdu, china                                                                                         july 2003

• dissertation title: the cloning and sequence analysis of hlf cdna.  advisor: dr. ying tong
• national computer rank examination, rank ii

 

~ professional affiliation ~

member, biophysics society                                                                                                                  2006 c present

research experience                       

oklahoma state university, stillwater, ok                                                                                                 august 2003 c present

supervising professor: dr. chang-an yu

• studied the supernumerary subunit subunit iv of cytochrome bc1 complex from rhodobacter sphaeroides, constructed different kinds of site-directed mutations, and identified the amino acid residues essential for its reconstitutive activity.

reference: tso, yin, et al. (2006) biochim. biophys. acta. 1757, 1561-1567

      

• studied the superoxide generation by bc1 complex, and elucidated the effect of subunit iv on the generation based on the results of kinetics and stopped flow analysis.

reference: yin, et al. (2009) biochim. biophys. acta. 1787(7):913-9

 

• utilized activity assays and superoxide generation determination; proposed a mechanism for the superoxide generation by bc1 complex.

reference: yin, et al. (2009) to be submitted soon.

 

• mentored three graduate students, supervised lab technician, and oversaw one undergraduate student engaged in membrane protein project.

 

continued

 

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research presentations                 

chang-an yu, xiaowei chen, maria elberry, buddha gurung, hewen ma, shaoqing yang, yyyyyy x. yyyyyy, fei zhou and linda yu (2007) structural and mechanistic studies on cytochrome bc1 complex , 63rd southwest regional meeting of the american chemical society, lubbock, tx.

 

yyyyyy x. yyyyyy, linda yu, chang-an yu, (2006) the putative metal binding site in cytchrome c1 of rhodobacter sphaeroides bc1 complex is involved in the interaction of the core complex and subunit iv , biophysical society 51^st annual meeting, baltimore, md.

publications                                      

yyyyyy x. yyyyyy, shih-chia tso, chang-an yu, linda yu, (2009) effect of subunit iv on superoxide generation by rhodobacter sphaeroides cytochrome bc1 complex , biochim. biophys. acta. 1787(7):913-9

 

yyyyyy x. yyyyyy, shaoqing yang, chang-an yu, linda yu, the reaction mechanism of superoxide generation during ubiquinol oxidation by the cytochrome bc1 complex , to be submitted soon.

 

linda yu, shaoqing yang, yyyyyy x. yyyyyy, xiaowei cen, fei zhou, di xia, chang-an yu, (2009) chapter 25 analysis of electron transfer and superoxide generation in the cytochrome cytochrome bc1 complex . methods enzymol. 456: 459-73.

     

chang-an yu, xiaowei cen, hewen ma, yyyyyy x. yyyyyy, linda yu, lothar esser, di xia, (2008) domain conformational switch of the iron-sulfur protein in cytochrome bc1 complex is induced by the electron transfer from cytochrome bl to bh . biochim. biophys. acta. 1777(7-8):1038-43

 

shih-chia tso, yyyyyy x. yyyyyy, chang-an yu, linda yu, (2006) identification of amino acid residues essential for reconstitutive activity of subunit iv of the cytochrome bc1 complex from rhodobacter sphaeroides , biochim. biophys. acta. 1757, 1561-1567.

 

 

 

 

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hiring agent name

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company name

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dear__________________:

 

i am currently seeking a challenging career opportunity in r&d capacity and am submitting my resume for your review.  in advance, thank you for your time and consideration.

 

as demonstrated in the accompanying resume, i recently completed my doctorate in biochemistry and molecular biology.  during the course of my studies, i gained valuable hands-on experience independently conducting protein research projects, including a long-term project in which i was entrusted with oversight of junior researchers.  i am adept at performing specialized research, conducting experiments, and preparing and delivering presentations on my findings.  my dynamic organizational and analytical skills have consistently proven instrumental in my success, additionally, i have excellent communication skills and am fluent in chinese.

 

as an employee, you will find me to be a driven team player committed to supporting you in achieving your objectives through superior performance.  i am confident that i could be a valuable asset to your organization, and look forward to interviewing with you in the near future.

 

sincerely,

 

 

yyyyyy x. yyyyyy